To improve exosome concentration and its quality, several technologies have been developed for exosome purification, among which ultracentrifugation
and microfiltration, size exclusion chromatography(SEC), and immunoaffinity capture are favored.
Until recently, the primary methods of assessing a patient's sdLDL-C levels were based on the laborious and time-consuming ultracentrifugation
and electrophoresis methods.
In contrasts to above finding literature Kamezaki F et al have concluded that direct measuring LCLc methods over estimates, rather than underestimates LDLc results.20 Chotkowska et al have also highlighted mLDLc methods once compared with gold standard technique of ultracentrifugation
to demonstrate a positive bias in comparison to Friedewald's equation.21 Taken together the contrasts in literature with regards to methodologies over and underestimating triglycerides, possible explanation could be: First of all, as the triglyceridemia affects LDLc measures in calculated methods which is evident by the use of different factors to manage VLDL in serum triglyceride calculation, so calculated methods are inherently biased to the presence of triglyceride adjustment.22,23
Based on the size and density of exosomes, we can collect exosomes by ultracentrifugation
at 20,000 xg .
Lipoproteins are classified by ultracentrifugation
into five major types, with increasing density and decreasing size: chylomicrons (CMs), very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL).
As previously reported , the tube-length distribution range can be controlled by ultracentrifugation
of the SWCNT dispersion.
We obtained a collection rate of over 99 percent, surpassing ultracentrifugation
as well as other methods that are currently being used in the field."
F4370), concentrated by ultracentrifugation
and purified by sucrose gradient ultracentrifugation
To isolate the specific signal for chylomicrons from other contaminants that may cause false-positive identification of a chylous effusion, lipid analysis can be performed on fractions of the body fluid after ultracentrifugation
that separates the more dense HDL/LDL fraction (the HL fraction, density > 1.006 g/L) from the less dense VLDL/chylomicron fraction (VL fraction, density <1.006 g/L) (7).
Beckman Coulter Life Sciences returns analytical ultracentrifugation
(AUC) to the center of protein research and macromolecule characterization with the next-generation Optima AUC.
Sedimentation Velocity Analytical Ultracentrifugation
: Discrete Species and Size-Distributions of Macromolecules and Particles