In the present study, to further explore the etiology of HPND, the diseased crabs with HPND were collected from Yangcheng Lake Modern Agriculture Development Company, Suzhou, China, observation of the ultrathin section
of tissues from the diseased crabs were observed with a transmission electron microscope and animal regression test were performed, moreover, toxic effect of avermectin and high pH water on crabs was also examined, the results indicated that HPND was not caused by virus or microsporidian and might be induced by water of high pH value and other environmental factors.
Because of a similar reason, it was practically difficult to trace distal processes of the immunopositive neurons as long as they may form synaptic contacts by serial ultrathin section
B) An electron photomicrograph of an ultrathin section
of the control rat's testis showing interstitial endocrine cells (Leydig) with large oval indented nucleus (N) with condensed heterochromatin and prominent nucleolus (NU).
of 70 nm thick were cut using a diamond knife.
(40 to 50 nm) of tissue samples were cut using a Leica Ultracut UCT ultramicrotome (Leica Company, Solms, Gemany).
Semithin and ultrathin sections
were obtained using an RMC MT-6000 ultramicrotome.
(5070 nm) were subsequently cut with a diamond knife on an LKB-11800 ultramicrotome (LKB Stockholm Sweden) and collected on 300- mesh copper grids.
After complete polymerization, the abscission zone tissues were obtained and made into ultrathin sections
After light microscopic orientation, 70 nm ultrathin sections
were cut and stained by uranyl acetate and lead citrate .
were cut with a Reichert Ultracut S ultramicrotome (Leica, Wien, Austria).
For ultrathin sections
the block face was first trimmed and the semithin sections were provided at 500 nm thicknesses, stained with 1% toluidine blue, and were examined by light microscopy, for finding the desired area.