Based on the present findings, formulating complementary foods using a blend of ungerminated
with germinated sorghum flour in the ratios of 75:25, respectively, might be a viable option, because germinated flour needs to be prepared less often.
After 28 d of incubation, abnormal seedlings were counted and ungerminated
seed were examined by tetrazolim (TZ, 2,3,5-triphenyl tetrazolium chloride) tests (AOSA, 1998) and classified as dormant or dead.
However, it is unlikely that this phenotypic effect of GA led to size differences between the natural and induced plants in this experiment for two reasons: (1) Most of the plants derived from naturally germinating seeds actually received GA, since GA was sprayed on all of the pots in the growth chamber 5 days after sowing (both ungerminated
, dormant seeds, and newly emerging "natural" seedlings that had not yet been transplanted out of the growth chamber and into the greenhouse), and most of the naturally emerging seedlings were still in the growth chamber at this time [ILLUSTRATION FOR FIGURE 1 OMITTED]; and (2) there were no significant differences in the height, number of leaves, and diameters of natural and induced plants in the field.
This method could not be used to quantify ungerminated
pollen grains because they may have been washed away during this treatment.
Sorghum, for example, is soaked, germinated and is eaten or ground to flour and added to ungerminated
cereal flour, reducing the cereal viscosity and activating endogenous cereal phytases that break down phytate into lower inositol phosphates.
After 28 d of incubation, ungerminated
seeds were examined by TZ tests and classified as dormant or dead.
achenes again were placed in respective salt solutions in a 5 C dark incubator for an additional 30 days on 30 January 1995 in another attempt to break their dormancy.
ponderosa seed markers not associated with seedlings in May, and 110 of these were found with ungerminated
seeds (Table 4).
Seed germination after three months is negligible and most ungerminated
seeds are dead.
The Petri dishes were covered and sealed with tape, wrapped in aluminum foil, and incubated at 28[degrees]C for 3 d, after which germinated and ungerminated
Viability of ungerminated
achenes was determined by pinching them with forceps under a microscope to see if they contained firm, white (viable) embryos or soft, light brown (nonviable) embryos.
spinosa has a short-lived seedbank: [approximate] 11.5% of ungerminated
seeds are still viable the next year.