Disease was recorded using 0-4 infection scale : (0) no uredia
or other macroscopic sign of infection, (;) no uredia
, but hypersensitive necrotic or chlorotic flecks present, (1) small uredia
surrounded by necrosis, (2) small to medium uredia
often surrounded by chlorosis; green islands may be surrounded by chlorotic or necrotic border, (x) random distribution of variable-sized uredia
on single leaf, (3) medium sized uredia
that may be associated with chlorosis, and (4) large uredia
stripes formed on the leaves at mature stage of the planted give yellow and dusty appearance.
Each race was originally purified by single spore isolation or by transfer of single uredia
for several generations.
The infection type was recorded as 6 classes (0 to 4) according to the methods also described by Luo et al (2005), respectively as follows: 0, no visible symptoms; 0;, few necrotic flecks; 1, small sporulating uredia
surrounded by necrotic tissue; 2, small to medium uredia
with chlorosis and necrosis; 3, moderately sized sporulating uredia
surrounded only by chlorotic tissue; and 4, abundantly sporulating uredia
([paragraph]) Rust reaction; 1 = immune, no pustules; 2 = small pustules; 3 = intermediate pustules uredia
less than 0.3 mm diameter, 4 = uredia
0.3 to 0.5 mm diameter, 5 = uredia
0.5 of 0.8 mm, and 6 = uredia
larger than 0.8 mm diameter (Stavely et al., 1983).
are present, generally with little or no chlorosis or necrosis.
in soybean leaflets treated with the EO of CI.
IT designations of plants refer to: 4 = extremely susceptible with large uredia
; 33- = moderately susceptible with combination of medium size (3) and small size (3-) uredia
; 0; = resistant with necrotic flecks; and 0;N = resistant with necrotic lesions.
Plants of the HA-R6, HA-R7, and HA-R8 germplasm populations had an immune reaction (no uredia
) to race 777.
The spreader rows were removed when the first uredia
were observed on the known susceptible cultivars in the trial (Table 2).