Some of the key players in the global fetal bovine serum market are HiMedia Laboratories, Merck KGaA, Bovogen Biologicals Pty Ltd, Atlanta Biologicals Inc., TCS Biosciences Ltd, Tissue Culture Biologicals, Rocky Mountain Biologicals, GE Healthcare, Thermo Fisher Scientific, Bio-Techne, PAN-Biotech, Access Biologicals, Animal Technologies Inc., Nucleus Biologics, Biological Industries, Corning Incorporated, J R Scientific Inc., Moregate Biotech, Wisent
Inc, Zhejiang Tianhang Biotechnology Co.
ADSCs were isolated from subcutaneous adipose tissue in the groin of New Zealand white rabbits (purchased from Wuxi Puhe Biomedical Co., Ltd., China) as performed previously. The ADSC primary cultures were obtained by enzymatic digestion with 0.1% collagenase Type I (Invitrogen, Thermo Fisher Scientific Inc., USA) and treated with 10% fetal bovine serum (FBS; Wisent
, Canada) to inactivate the collagenase.
Fetal bovine serum (FBS) and other cell culture reagents were from Wisent
(Quebec, QC Canada).
All cell lines placed in a humidified incubator with 5% carbon dioxide (CO2) were cultured in Dulbecco's modified Eagle's medium (DMEM) (Multicell; Wisent
, Saint-Bruno, Quebec, Canada) with 10% foetal bovine serum (FBS), 50 U/mL penicillin, and 50 mg/mL streptomycin (Gibco, Carlsbad, California (CA), Unites States) at 37AdegC.
Briefly, human primary preadipocytes were seeded in sixwell dishes in subcutaneous preadipocyte media (PM-1, Zenbio Inc.), containing 10% fetal calf serum (Wisent
, Montreal, Quebec, Canada).
Dulbecco's modified eagle medium (DMEM F-12), fetal bovine serum (FBS), phosphate buffered saline (PBS) and antibiotic solutions from Multicell Wisent
(Quebec, Canada); Annexin V-FITC apoptosis detection kit with propidium iodide (PI) from Biolegend (San Diego, CA, USA); the other chemicals from Merck (NJ, USA) were purchased.
(2006) determined the productivity and cost changes of five different cut-to-length logging concepts (a Ponsse Wisent
dual concept, three Valmet combi concepts, and a harvester--forwarder chain concept).
Cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM), fetal bovine serum (FBS), phosphate buffered saline (PBS, 10X) and antibiotic solutions were purchased from Multi cell Wisent
This story does not stop at the North American plains subspecies (Bison bison bison), but it also applies to the North American woods bison subspecies (Bison bison athabascae) along with the European bison species (Bison bonasus; wisent
After a centrifugation step at 900 g x 20 min at room temperature on a lymphocyte separation medium cushion (density, 1.077g/ml) (Wisent
), PMNL were obtained (>96%) followed by hypotonic lysis of the remaining erythrocytes.
EBM-2 media without growth factors (Lonza) with 50 mM HEPES (Wisent
) was adjusted to either pH 7.4 or 6-6.7 using HCl.
HSCs were cultured in Dulbecco's modified eagle medium (DMEM; Invitrogen, Grand Island, NY, USA) with 10% fetal bovine serum (FBS; Wisent
Biotechnology Co., Ltd., Nanjing, China), 1% antibiotics, and grown in a 5% C[O.sub.2] humidified atmosphere at 37[degrees]C.