The histochemical stains used were: Xylidine
Ponceau (Melo and Vidal, 1980) and Bromophenol Blue (Pearse, 1985) for the detection of proteins; Periodic acid-Schiff (PAS) and simultaneous PAS and Alcian Blue (Junqueira and Junqueira, 1983) for polysaccharides; vonKossa method (Junqueira and Junqueira, 1983) for calcium detection; and Nile Blue (Lison, 1960) and Sudan Black B (Junqueira and Junqueira, 1983) for lipids detection.
The toxic properties of xylidine
The film was cut in 1[cm.sup.2] and stained with Xylidine
Ponceau solution for 30min, then washed in a bath of acetic acid 2.5% for 10min and finally washed in a distilled water bath for 10min The stained film was placed on a glass microscope slide and dried in an oven for 24h at 37[degrees]C.
The antibacterial results manifestly exhibited that N-sulfonated derivatives of 2,3- xylidine
possess a comprehensive range of activity against different bacterial strains.
For histochemistry, the SR sections were stained for proteins using the Xylidine
ponceau (Mello and Vidal, 1980) and mercuric-bromophenol blue (Pearse, 1985) techniques.
The addition of inducers such as xylidine
, phenolic mixture, and copper for the white-rot fungi T versicolor and in Pleurotus ostreatu has been reported for augmentation in laccase production.
The anatomical sections were stained with 0.05 % Toluidine blue (O'Brien et al., 1964), Sudan Black B to detect lipids (Jensen, 1962), PAS reagent to detect polysaccharides (O'Brien & McCully, 1981), Aniline Blue Black (Fisher, 1968) and Xylidine
Ponceau (Vidal, 1970) to detect proteins.
The original method of Masson (1928) was initially thought for carcinoids analysis, integrating four dyes (Regaud's hematoxylin, acid fuchsin with Ponceau de xylidine
and aniline blue) which have been repeatedly combined on later modifications.
The concentration of xylidine
is decreasing rapidly, at the same time TOC decreases as part of the organic carbon is destroyed.