Briefly, the ruminal samples were removed from the formalin, trimmed, and dehydrated thorough a graded ethanol series, immersed in xylol
, and embedded in paraffin wax.
The samples were processed in a Histokinette Leica TP-1020 with different grades of alcohol, acetone, and xylol
After six ethanol (from 70%-100%) and xylol
baths, they were embedded in paraffin.
After one-night incubation at 37-40[degrees]C, dewaxing of the 4 [micro]m sections was completed by another 45 minutes of incubation at 65[degrees]C and immersion in xylol
for 20 minutes.
In the remaining four experimental groups, the canals were emptied using PTR alone (group 2), with the addition of the solvent xylol
(Bioquimica Industria Farmaceutica, Sao Jose do Rio Preto, SP, Brazil) (group 3), orange oil (Citrol; Biodinamica Quimica and Farmaceutica, Ibipora, PR, Brazil) (group 4), and eucalyptol (ASFER Industria Quimica, Sao Caetano do Sul, SP, Brazil) (group 5).
Samples were dehydrated in ethyl alcohol and xylol
for one hour, and were embedded in paraffin.
Tenders are invited for procurement of xylol
for assam asset
0 g) of each gonad was removed, dehydrated in ethanol, cleared in xylol
, and embedded in paraffin.
After fixation, the samples were dehydrated in ascending concentrations (70, 80, 90 and absolute) of ethyl alcohol, followed by diaphanization with xylol
and inclusion in paraffin.
Light microscopy: The fixed ovaries from cultured (n=5 in each group) and non-cultured groups (n=5 for 14 day mice and n=5 for 21 day mice) by Bouin's solution were dehydrated in a graded series of ethanol and cleared with xylol
and finally embedded in paraffin wax.
The dehydrated specimens were cleared in xylol
and then embedded in paraffin wax using 'L' blocks.
Liver tissue purification process has been carried out in the next stage of xylol
and paraffin embedding.