A comparison of photographic analyses used to quantify zooxanthella
density and pigment concentrations in cnidarians.
KEY WORDS: tridacnids, zooxanthella
, symbiotic relationship, strains, culture condition, Japanese seed production, Symbiodinium
28) This zooxanthella
takes in carbon dioxide (C[O.
Spatial and temporal photoacclimation of Stylophora pistillata: zooxanthella
size, pigmentation, location and clade.
In addition, if zooxanthellae do contribute substantially to the C budget of the host sponge, we hypothesize that this contribution is also influenced by the habitat in which the sponge lives, as the reduced light levels and the reduced zooxanthella
concentrations (Hill, 1999) of deeper locations force the host sponges to rely more heavily on heterotrophy.
For the analysis of temporal variation, each sample was homogenized in zooxanthella
isolation buffer (ZIB) (Rowan and Powers, 1991) and an aliquot taken for protein quantification using the micro-assay of the Bio-Rad Protein Assay (Bio-Rad Laboratories, Hemel Hempstead.
Since zooxanthellae are larger and therefore contain 3 (summer) and 2 (winter) times the carbon content of zoochlorellae (Table 1), a dividing zooxanthella
requires more carbon for growth.
Interspecific comparisons are important because corals may react differently to a change in their environmental conditions resulting from different levels of oxidative stress (Lesser, 1996), zooxanthella
genotypes (Rowan et al.
Percent infection was determined by taking a subsample of 100 larvae from each bowl and examining the larvae with light microscopy to quantify the proportion containing at least one zooxanthella
densities are below approximately 2 X [10.
The blastomeres were now 20-30 /[mu]m in diameter, even in size, and rarely contained more than one zooxanthella
Members of the species are densely populated with the zooxanthella
, Symbiodinium sp.