zymography


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zy·mog·ra·phy

 (zī-mŏg′rə-fē)
n.
An electrophoretic technique used to detect the identity and activity of a protease in a sample or tissue, in which the protease is allowed to react with a protein substrate, as in a gel, and the products of the reaction are identified with stains or by other means.

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References in periodicals archive ?
Purified trypsin showed a single band on both SDS and zymography (Figure 2a, b) suggesting their high purity and showing only one dominant isoform of trypsin in Dosidicus gigas hepatopancreas.
3 shows the activity of laccase on SDS-PAGE and the laccase enzyme was successfully oxidizing ABTS substrate to produce two dark green bands for enzyme zymography.
Zymography is a valuable tool for the visualization of proteolytic activity on an electrophoretic gel matrix, by using a given protein substrate [25].
To detect metalloproteinase activity, gelatin zymography was performed by the method described by Unemori and Werb [19].
Fine-structural distribution of MMP-2 and MMP-9 activities in the rat skeletal muscle upon training: a study by high- resolution in situ zymography.
Mannello, "Effects of blood collection methods on gelatin zymography of matrix metalloproteinases," Clinical Chemistry, vol.
The enzymatic activity of MMPs was assessed by zymography and was enhanced in higher-grade OSCC and along the advancing tumor front.
For MMPs activity assay, fluorescence resonance energy transfer-based MMPs activity assay and gelatin zymography assay were involved in this study.
As TIMP2 is required for the efficient activation of proMMP2 by MT1-MMP, immunohistochemistry [79] and in situ zymography [79] demonstrated their co-localization in epithelial carcinomatous and adjacent stromal cells.