Testicular cell number estimation: To estimate the total number of spermatogonia A (pale-staining nucleus with a fine "dusty" distribution of heterochromatin throughout the nucleus), B (with dense clumps of heterochromatin around the periphery of the nucleus), (24) Sertoli cells, spermatocytes, round and long
spermatids, and Leydig cells, a Nikon E200 light microscope (Tokyo, Japan) fitted with a 60x oil objective lens was applied at the final magnification of 1,640x.
The organ maintains a continuous supply of
spermatids by nurturing germ cells.
D1 and D2 were taken only when D1/D2 [less than or equal to] 0.85, others include epithelial height, testicular weight, sustentacular cells (Sertoli cells) to germ cell ratio (elongated
spermatids) (Qin & Lung, 2002).
In the control group, the rat testes showed normal morphology and spermatogenesis, healthy seminiferous tubules containing plenty of
spermatids and sperm in the lumen (Figure 1).
In the evaluation of the spermatogenesis stages, 100 sections of the seminiferous tubules of animals' testis in puberty and sexual maturity, were classified into stages of I-VI (presence of two generations of
spermatids), VII-VIII (mature
spermatids present in lumen), IV-XII (presence of a
spermatid generation) XIV (presence of secondary spermatocytes) (Ferreira, Lison, & Valeri, 1967).
initiation of meiosis and formation of
spermatids might be regulated by high concentration of T during May and June as observed in other teleosts including catla (Bhattacharyya and Maitra, 2006).
Category 9 denotes many end-stage
spermatids, value 8 less than five spermatozoa and few late-stage
spermatids.
Meanwhile, ROSI is a unique technique of injecting a female's eggs with immature sperm cells -
spermatids - which can be found in the testes of men who would normally be pronounced sterile.
Additionally, disordered Sertoli-germ cell adhesion disrupts germ cell localization and causes premature release of
spermatids into the lumen, thus resulting in an abnormal elongation process and infertility [18].
Primary spermatocytes predominate in early recrudescence whereas in late recrudescence, secondary spermatocytes and
spermatids were abundant; (3) Spermiogenesis, lumina of seminiferous tubules were lined by sperm or clusters of metamorphosing
spermatids.
For these purposes, there are two methods to characterize stages of the seminiferous epithelium cycle: (1) based on tubular morphology and (2) based on development of the acrosomic system and nuclear morphology of developing
spermatids.
Spermatids were characterized by a change in cell shape associated with the elongation of the nuclei and were smaller than spermatocytes (Fig.