These fertilizers also make nitrogen available for legume crops by fixing atmospheric nitrogen in the soil and their root nodules. They also enhance the fertility of the soil and improve the yield significantly, without adversely affecting the environment.
Leguminous crops (Fabaceae family) improve soil fertility through nitrogen fixation with the help of rhizobia bacteria, which are found in their root nodules.
According to Garbaye and Frey-Klett [1], it is well established that some strains of Rhizobium can exhibit nitrogenase activity when grown in vitro on different media, but such activity has not been reported for Actinomycetes strains isolated from root nodules of N2-fixing nonlegumes [1].
Mo is a constituent of nitrogenase, a key enzyme in all [N.sub.2] fixing microorganisms; consequently, nitrogen-fixing plants have a high Mo requirement, particularly in the root nodules (Hille, 1999; Marschner, 2012).
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